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In vivo Visualization of Synaptic Vesicles Within Drosophila Larval Segmental Axons
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神经科学
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JoVE 杂志 神经科学
In vivo Visualization of Synaptic Vesicles Within Drosophila Larval Segmental Axons

In vivo Visualization of Synaptic Vesicles Within Drosophila Larval Segmental Axons

DOI:
10.3791/2151-v

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05:58 min

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October 15, 2010

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1Department of Biological Sciences,SUNY-University at Buffalo

Chapters

  • 00:00Title
  • 00:49Introduction
  • 01:01Preparation for Dissection
  • 01:51Collection of Larvae
  • 02:16Live Dissection of Larvae
  • 04:18Live Imaging of Dissected Larvae
  • 05:00Representative Images
  • 05:37Conclusion

Summary

自动翻译

自动翻译

This protocol discusses the live dissection of Drosophila larvae for the purpose of imaging the movement of GFP tagged axonal vesicles on microtubule tracks.

Tags

In Vivo VisualizationSynaptic VesiclesDrosophila Larval Segmental AxonsAxonal TransportNeurological DiseasesDissection ProtocolLive ImagingUAS-GAL4 MethodGFP-tagged APPSynaptotagmin VesiclesNeuronal GAL4 DriversFluorescently Tagged MarkersMitochrondriaMitoTrackerLysosomesLysoTrackerGFP-vesicle MovementParticle Movement

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