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Kvantifiering av proteiner med Peptide Immunoaffinity Anrikning kombination med masspektrometri
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Kvantifiering av proteiner med Peptide Immunoaffinity Anrikning kombination med masspektrometri

Article DOI: 10.3791/2812-v • 06:09 min • July 31st, 2011
July 31st, 2011 •
*1, *1, 2, 3, 4, 5, 2, 3, 1
1Clinical Research Division, Fred Hutchinson Cancer Research Center - FHCRC, 2Department of Biochemistry and Microbiology, University of Victoria, 3Broad Institute of MIT and Harvard, 4Genome BC Proteomics Centre, University of Victoria, 5Plasma Proteome Institute
* These authors contributed equally

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Stabila isotopen standarder och Capture av Anti-peptidantikroppar (SISCAPA) par affinitet anrikning av peptider med stabila isotoputspädning masspektrometri (MRM-MS) för att ge kvantitativ mätning av peptider som substitut för sina respektive proteiner. Här beskriver vi det protokoll som använder magnetiska partiklar i en delvis automatiserad format.

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Molekylärbiologi masspektrometri målinriktad analys peptid MRM SISCAPA protein kvantifiering
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