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Eksperimentel Generering af Carcinoma-associerede fibroblaster (cafeer) fra Human brystkirtler fibroblaster

Article DOI: 10.3791/3201-v • 15:43 min • October 25th, 2011
October 25th, 2011 •
*1, *1, 1,2
1CR-UK Stromal-Tumour Interaction Group, Paterson Institute for Cancer Research, University of Manchester, 2Atopy Research Center, Juntendo University
* These authors contributed equally

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Carcinoma-associeret fibroblaster (cafeer) rig på myofibroblasts stede i tumor stroma, spiller en vigtig rolle som drivkraft for tumorprogression. Vi udviklede en coimplantation tumor xengraft model for eksperimentelt at generere cafeer fra menneskelige brystkirtler fibroblaster. Protokollen beskriver, hvordan man etablerer CAF myofibroblasts, der erhverver en evne til at fremme tumorgenese.

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Medicin kræft stromale myofibroblasts eksperimentelt genereret carcinoma-associerede fibroblaster (exp-cafeer) fibroblast menneskelige brystkirtler carcinomer tumorxenoplantater
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