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Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond

Article DOI: 10.3791/50484-v • 09:00 min • July 27th, 2013
July 27th, 2013 •
1, 2, 1, 1, 1
1Signalling Programme, The Babraham Institute, 2MRC Group, Cardiff School of Biosciences, Cardiff University

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Zeitraffer-Mikroskopie von fluoreszenzmarkierten Autophagie Marker erlaubt die Überwachung der dynamischen Autophagie Reaktion mit hoher zeitlicher Auflösung. Mit spezifischen Autophagie und Organellen Marker in einer Kombination aus 3 verschiedenen Farben, können wir folgen den Beitrag eines Proteins an Autophagosom Bildung in einem robusten räumlichen und zeitlichen Kontext.

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Cellular Biology Ausgabe 77 Molekularbiologie Biochemie Phosphatidylinositole Mikroskopie Fluoreszenz- Video Autophagie Zellbiologie Autophagie Omegasome DFCP1 LC3 Live-Bildgebung Zeitraffer-Mikroskopie Zelle Imaging
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