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Intravital mikroskopi af Imaging Subcellulære Strukturer Live mus, der udtrykker fluorescerende proteiner

Article DOI: 10.3791/50558-v • 08:23 min • September 1st, 2013
September 1st, 2013 •
1,2, 1, 1, 1,3, 1
1Intracellular Membrane Trafficking Unit, Oral and Pharyngeal Cancer Branch National Institute of Dental and Craniofacial Research, National Institutes of Health, 2Department of Biology, University of North Carolina at Chapel Hill, 3Department of Chemical & Biochemical Engineering and Department of Biomedical Engineering, Rutgers University

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Intravital mikroskopi er et kraftfuldt værktøj, der giver billeddannelse forskellige biologiske processer i levende dyr. I denne artikel præsenterer vi en detaljeret metode til billeddannelse dynamikken i subcellulære strukturer, såsom de sekretoriske granulat, i spytkirtlerne af levende mus.

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Cellebiologi mikroskopi konfokal mikroskopi fluorescens multiphoton exocytose cellebiologi dyr biologi dyremodeller Intravital Microscopy Spytkirtler exocytose In Vivo Imaging
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