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JoVE Journal Chemistry
Isolering och kemisk karakterisering av lipid A från gramnegativa bakterier
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Isolering och kemisk karakterisering av lipid A från gramnegativa bakterier

Article DOI: 10.3791/50623-v • 12:57 min • September 16th, 2013
September 16th, 2013 •
1, 2, 2, 1,3
1Section of Molecular Genetics and Microbiology, The University of Texas at Austin, 2Department of Chemistry and Biochemistry, The University of Texas at Austin, 3The Institute of Cellular and Molecular Biology, The University of Texas at Austin

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Isolering och karakterisering av lipid A-domänen av lipopolysackarid (LPS) från gramnegativa bakterier ger en inblick i cellytan baserade mekanismer för antibiotikaresistens, bakteriell överlevnad och fitness, och hur kemiskt varierande lipid A molekylslag modulera differentiellt värd medfödda immunsvar.

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Chemistry membranlipider Toll-like receptors Endotoxiner glykolipider lipopolysackarider lipid A mikrobiologi Lipids lipid A Bligh-Dyer tunnskiktskromatografi (TLC) en lipopolysackarid masspektrometri Collision Induced Dissociation (CID ) Photodissociation (PD)
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