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Ricostituzione della β-catenina degradazione nel

Article DOI: 10.3791/51425-v • 09:41 min • June 17th, 2014
June 17th, 2014 •
*1, *1, 2, 1,3
1Department of Cell and Developmental Biology and Program in Developmental Biology, Vanderbilt University Medical Center, 2Division of Gastroenterology, Hepatology & Nutrition and Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, 3Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine
* These authors contributed equally

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Un metodo è descritto per l'analisi degradazione delle proteine ​​usando radioattivo e proteine ​​luciferasi-fusione in estratto uovo Xenopus e il suo adattamento per lo screening high-throughput per piccoli modulatori molecola di degradazione delle proteine.

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Biologia Molecolare Numero 88, la degradazione delle proteine radiomarcatore luciferasi autoradiografia screening ad alto rendimento
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