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Chapters
- 00:05Title
- 01:24Fixation
- 03:58Rehydration, Sample Preparation, and Sonication
- 06:16Immunostaining
- 08:12Results: Dynamic Images with Sonication-based Immunostaining
- 09:20Conclusion
Immunostaining is an effective technique for visualizing specific cell types and proteins within tissues. By utilizing sonication, the protocol described here alleviates the need to dissect Drosophila melanogaster tissues from late-stage embryos and larvae before immunostaining. We provide an efficient methodology for the immunostaining of formaldehyde-fixed whole mount larvae.
