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Echtzeit-Bildgebung von Einzel Engineered RNA-Transkripte in lebenden Zellen Mit Ratiometrische Bimolekulare Beacons

Article DOI: 10.3791/51544-v • 12:20 min • August 6th, 2014
August 6th, 2014 •
*1, *1, 2, 2, 1
1Department of Bioengineering, University of Pennsylvania, 2Integrated DNA Technologies, Inc.
* These authors contributed equally

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Ratiometrische bimolekularen Beacons (RBMBs), um die Bild einzigen entwickelt RNA-Transkripte in lebenden Zellen verwendet werden. Hier beschreiben wir die Herstellung und Reinigung von RBMBs, Lieferung RBMBs in Zellen von Mikroporation und fluoreszierende Abbildung einzelner RNA-Transkripte in Echtzeit.

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Genetik RNA Bildgebung Einzelmolekül- Fluoreszenz lebende Zelle
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