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Combined In vivo Optical and µCT Imaging to Monitor Infection, Inflammation, and Bone Anatomy in an Orthopaedic Implant Infection in Mice
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Combined In vivo Optical and µCT Imaging to Monitor Infection, Inflammation, and Bone Anatomy in an Orthopaedic Implant Infection in Mice

Article DOI: 10.3791/51612-v • 18:40 min • October 16th, 2014
October 16th, 2014 •
1, 2, 2, 3, 3, 1, 2, 3,4
1Orthopaedic Hospital Research Center, Orthopaedic Hospital Department of Orthopaedic Surgery, David Geffen School of Medicine at University of California, Los Angeles (UCLA), 2PerkinElmer, 3Department of Dermatology, Johns Hopkins University School of Medicine, 4Department of Medicine, Division of Infectious Diseases, Department of Orthopaedic Surgery, Johns Hopkins University School of Medicine

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Combined optical and μCT imaging in a mouse model of orthopaedic implant infection, utilizing a bioluminescent engineered strain of Staphylococcus aureus, provided the capability to noninvasively and longitudinally monitor the dynamics of the bacterial infection, as well as the corresponding inflammatory response and anatomical changes in the bone.

Tags

In Vivo Optical Imaging µCT Imaging Infection Monitoring Inflammation Monitoring Bone Anatomy Orthopaedic Implant Infection Multimodality Imaging Bacterial Biofilms Persistent Inflammation Osteomyelitis Osteolysis Implant Loosening Implant Failure Mouse Model Kirschner-wire Implant LysEGFP Mice Bioluminescent Imaging Fluorescent Imaging
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