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Chapters
- 00:05Title
- 01:49Cell Culture
- 02:24Isolation of Mitochondria from Cultured Cells
- 04:58Mitochondria Isolation from Mouse Tissue
- 06:26Assessment of Mitochondrial (Dys)function
- 07:42Results: Cytokine Treatment Leads to Increased Cell Death and Decreased Membrane Potential in Mitochondria
- 08:51Conclusion
Comparison of mitochondrial membrane potential between samples yields valuable information about cellular status. Detailed steps for isolating mitochondria and assessing response to inhibitors and uncouplers using fluorescence are described. The method and utility of this protocol are illustrated by use of a cell culture and animal model of cellular stress.
