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Base-Probe Real-time PCR Approcci per la misurazione quantitativa dei microRNA
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Base-Probe Real-time PCR Approcci per la misurazione quantitativa dei microRNA

Article DOI: 10.3791/52586-v • 10:28 min • April 14th, 2015
April 14th, 2015 •
1, 1, 1, 2, 1
1Diabetes and Islet Biology Group, NHMRC Clinical Trials Centre, Faculty of Medicine, The University of Sydney, 2Biomarkers Laboratory, NHMRC Clinical Trials Centre, Faculty of Medicine, The University of Sydney

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MicroRNA circolanti sono recentemente emersi come biomarcatori promettenti e innovativi per vari tipi di cancro e di altre malattie. L'obiettivo di questo articolo è quello di discutere di tre diverse piattaforme di PCR real-time sonda-based e metodi che sono disponibili per quantificare e determinare l'abbondanza dei microRNA circolanti.

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Biologia Molecolare microRNA ncRNA saggi sonda-based high-throughput PCR nanofluidica / Arrays Open reverse-trascrizione pre-amplificazione qPCR
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