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Las proteínas Análisis de inmunofluorescencia de endógenos y exógenos Centrómero-cinetocoro
JoVE 杂志
生物学
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JoVE 杂志 生物学
Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
DOI:

05:35 min

March 03, 2016

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Chapters

  • 00:05Title
  • 00:46Transfection and Immunofluorescent Staining for Endogenous Centromere Kinetochore Protein Detection
  • 03:30Results: Representative Immunoflurescence Analysis of Centromere Protein-A (CENP-A) Protein Expression
  • 04:42Conclusion

Summary

自动翻译

Here we report protocols to detect endogenous and exogenous centromere-kinetochore proteins in human cells and quantify these protein levels at centromeres-kinetochores by indirect immunofluorescent staining through the use of fixation (paraformaldehyde, acetone, or methanol fixation).

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