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JoVE Journal Biology
Isolering af CD146
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Isolering af CD146

Article DOI: 10.3791/53782-v • 09:47 min • June 17th, 2016
June 17th, 2016 •
1,2, 1,3,4, 1,2,5
1Sinclair Centre for Regenerative Medicine, Ottawa Hospital Research Institute, 2University of Ottawa, 3Department of Neonatology and Pediatric Critical Care Medicine, Medical Faculty and University Hospital Carl Gustav Carus, Technische Universität Dresden, 4DFG Research Center and Cluster of Excellence for Regenerative Therapies (CRTD), Technische Universität, Dresden, 5Children's Hospital of Eastern Ontario Research Institute

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Denne protokol beskriver en isolation teknik til opnåelse primære lunge residente mesenchymal stromaceller fra rotter, ved brug af enzymatisk fordøjelse, gradient separation massefylde, plast adhærens og CD146 + magnetiske perler markering.

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Stem Cell Biology Mesenchymale stromaceller væv resident stamceller / progenitorceller lunge rotte regenerativ medicin magnetisk perle udvælgelse
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