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1Division of Urologic Surgery,Washington University in St. Louis School of Medicine, 2Department of Urology,University of Wisconsin School of Medicine and Public Health, 3Department of Pathology and Laboratory Medicine,University of Wisconsin School of Medicine and Public Health, 4O’Brien Urology Research Center,University of Wisconsin School of Medicine and Public Health
Chapters
- 00:05Title
- 00:37Automated Image Acquisition and Analysis
- 01:43Tissue Segmentation
- 03:48Cell Segementation
- 05:16Scoring and IHC and Co-localization
- 05:50Applying the Algorithm and Batch Analysis
- 07:08Results: Successful Cell and Tissue Segmentation
- 08:18Conclusion
Immunohistochemistry is a powerful lab technique for evaluating protein localization and expression within tissues. Current semi-automated methods for quantitation introduce subjectivity and often create irreproducible results. Herein, we describe methods for multiplexed immunohistochemistry and objective quantitation of protein expression and co-localization using multispectral imaging.
