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Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Article DOI: 10.3791/54327-v • 10:27 min • July 10th, 2016
July 10th, 2016 •
1, 2, 2, 2,3, 1
1Lee Kong Chian School of Medicine, Nanyang Technological University, 2School of Chemical and Biomedical Engineering, Nanyang Technological University, 3NTU-Northwestern Institute of Nanomedicine, Nanyang Technological University

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This protocol describes the use of an inertial microfluidics-based buffer exchange strategy to purify micro/nanoparticle engineered cells with efficient depletion of unbound particles.

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Microfluidic Buffer Exchange Interference-free Micro/nanoparticle Cell Engineering Purification Technology Nanoparticles Separation Cell Laboring Procedure Ses-fa-tion Size-based Separation Inertial Focusing Effects Spiral Micro-device Regenerative Medicine Latch Volume Cell Processing Cell Engineering Field Imaging Purpose Function Control Hui Min Tay Doctor David Yeo Mesenchymal Stem Cells Confluency Six Well Plate Labeling Cells Silica Nanoparticles Calcium Dye Solution
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