Here, we present a protocol for isolating and culturing single cells with a microfluidic platform, which utilizes a new microwell design concept to allow for high-efficiency single cell isolation and long-term clonal culture.
Lin, C., Chang, H., Hsu, C. A Microfluidic Platform for High-throughput Single-cell Isolation and Culture. J. Vis. Exp. (112), e54105, doi:10.3791/54105 (2016).