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Chapters
- 00:05Title
- 00:35Dissection of Drosophila Ovaries
- 01:30Preparation for Live-tissue Microscopy
- 02:40Fluorescence Loss in Photobleaching Assay to Assess Mitochondrial Matrix Continuity
- 03:34Live Staining with Fluorescent Mitochondrial Dyes
- 05:13Co-immunostaining for Cyclin E and Mitochondria
- 07:48Results: Live Microscopy of the Drosophila Ovary
- 09:19Conclusion
Analysis of the mitochondrial structure-function relationship is required for a thorough understanding of the regulatory mechanisms of mitochondrial functionality. Specific methods for studying mitochondrial structure and function in live and fixed Drosophila ovaries are described and demonstrated in this paper.
