An Efficient and Flexible Cell Aggregation Method for 3D Spheroid Production

Sarah M. Maritan; Eric Y. Lian; Lois M. Mulligan

doi:10.3791/55544

Journal

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生物学

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三维球体制作一个高效，灵活细胞聚集方法

An Efficient and Flexible Cell Aggregation Method for 3D Spheroid Production

JoVE 杂志
生物学

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JoVE 杂志生物学

An Efficient and Flexible Cell Aggregation Method for 3D Spheroid Production

三维球体制作一个高效，灵活细胞聚集方法

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23,887 Views

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07:46 min

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March 27, 2017

DOI:

10.3791/55544-v

DOI:

10.3791/55544-v

•

07:46 min

March 27, 2017

•

23763 Views

Sarah M. Maritan*^1,2, Eric Y. Lian*^1,2, Lois M. Mulligan^1,2

¹Division of Cancer Biology and Genetics,Queen’s University Cancer Research Institute ²Department of Pathology and Molecular Medicine,Queen’s University

Summary

Here, we describe a rapid and flexible protocol for the formation of 3D cell spheroids through cell aggregation. This is easily adapted to multiple cell types and is suitable for use in a variety of applications including cell migration, invasion, or anoikis assays, and for imaging and quantifying cell-matrix interactions.