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Bioengineering

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JoVE Journal Bioengineering
Akış Sitometresi ile Primer Sıçan Retinalı Ganglion Hücrelerinin (RGC) İzolasyonu
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Characteristics of Precipitation-formed Polyethylene Glycol Microgels Are Controlled by Molecular Weight of Reactants
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Akış Sitometresi ile Primer Sıçan Retinalı Ganglion Hücrelerinin (RGC) İzolasyonu

Article DOI: 10.3791/55785-v • 11:01 min • July 5th, 2017
July 5th, 2017 •
1, 1, 1, 1, 1, 2, 1,3,4, 1,2
1Department of Ophthalmology, Hamilton Eye Institute, University of Tennessee Health Science Center, 2Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, 3Department of Anatomy and Neurobiology, University of Tennessee Health Science Center, 4Department of Pharmaceutical Sciences, University of Tennessee Health Science Center

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Milyonlarca insan irreversibl körlüğe neden olan retinal dejeneratif hastalıklardan muzdarip. Bu hastalıkların birçoğunun ortak bir unsuru retinal gangliyon hücrelerinin kaybıdır (RGC'ler). Bu ayrıntılı protokol, primer murin RGC'lerin akış sitometrisi ile pozitif ve negatif seçilim yoluyla izole edilmesini açıklar.

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Biyomühendislik Sayı 125 retina gangliyon hücreleri RGC akış sitometrisi nörodejenerasyon glokom yaşlanma
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