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Journal / Bioengineering / Y-27632 Kullanarak Birincil İnsan Dişeti Epitel…
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JoVE Journal Bioengineering
Y-27632 Kullanarak Birincil İnsan Dişeti Epitel Hücrelerinin İzolasyonu ve Kültürü
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Y-27632 Kullanarak Birincil İnsan Dişeti Epitel Hücrelerinin İzolasyonu ve Kültürü

Article DOI: 10.3791/62978-v • 06:47 min • November 6th, 2021
November 6th, 2021 •
1,2,3, 4, 3, 1, 1, 1, 1, 1, 1, 2,5, 1
1Department of Tissue Engineering and Regeneration, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, 2Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, 3Department of Stomatology, Shengli Oilfield Central Hospital, 4Department of Pediatric Surgery, Shengli Oilfield Central Hospital, 5Ningbo Stomatology Hospital of Savaid Stomatology School, Hangzhou Medical College

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Burada geleneksel yönteme Kaya inhibitörü Y-27632 eklenerek insan dişeti epitel hücrelerinin izolasyonu ve kültürü için değiştirilmiş bir yöntem sunuyoruz. Bu yöntem daha kolay, daha az zaman alıcıdır, kök hücre özelliklerini geliştirir ve hem laboratuvar hem de klinik uygulamalar için daha fazla sayıda yüksek potansiyelli epitel hücresi üretir.

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Biyomühendislik Sayı 177 diş eti epitel hücreleri enzimatik yöntem doğrudan eksplant yöntemi hücre izolasyonu hücre kültürü Y-27632 Rho ilişkili kinaz inhibitörü immünofluoresans analizi
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