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Vurdering af Sarcoplasmic Reticulum Calcium Reserve og intracellulære diastolisk Calcium fjernelse i isolerede ventrikulær Cardiomyocytes
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Vurdering af Sarcoplasmic Reticulum Calcium Reserve og intracellulære diastolisk Calcium fjernelse i isolerede ventrikulær Cardiomyocytes

Article DOI: 10.3791/55797-v • 11:00 min • September 18th, 2017
September 18th, 2017 •
*1, *2, 1, 1, 3, 1, 1
1Department of Cardiology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, 2Experimental Research Center, China Academy of Chinese Medical Sciences, 3Department of Cardiology, The Third Hospital of Hangzhou City
* These authors contributed equally

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Intracellulære calcium genanvendelse spiller en afgørende rolle i reguleringen af systolisk og diastolisk funktion i cardiomyocytes. Her, beskriver vi en protokol for at vurdere sarcoplasmic reticulum Ca2 + reserve og diastolisk calcium fjernelse funktion i cardiomyocytes af en calcium imaging system.

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Cellebiologi sag 127 Sarcoplasmic reticulum calcium homeostase calcium reserve intracellulære calcium fjernelse SERCA cardiomyocytes
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