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JoVE Journal Developmental Biology
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En RANKL-baserade Osteoclast kultur analys av mus benmärgen att undersöka betydelsen av mTORC1 Osteoclast bildandet

Article DOI: 10.3791/56468-v • 09:37 min • March 15th, 2018
March 15th, 2018 •
*1, *2, *1, 3, 2, 1, 2, 1
1Department of Pediatric Dentistry, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Center of Stomatology, 2State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, 3Department of Oral and Maxillofacial-Head and Neck Oncology, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine
* These authors contributed equally

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Detta manuskript beskriver ett protokoll till isolat och kultur osteoklaster i vitro från mus benmärg, och att studera rollen däggdjur/mekanistiska målet på rapamycin komplexa 1 i osteoclast formation.

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Utvecklingsbiologi fråga 133 Osteoclast mTORC1 Raptor ben remolding cellbiologi RANKL M-CSF
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