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Journal / Developmental Biology / RANKL による破骨細胞文化アッセイのマウス骨髄破骨細胞形成における mTORC1…
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JoVE Journal Developmental Biology
RANKL による破骨細胞文化アッセイのマウス骨髄破骨細胞形成における mTORC1 の役割を調査するには
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RANKL による破骨細胞文化アッセイのマウス骨髄破骨細胞形成における mTORC1 の役割を調査するには

Article DOI: 10.3791/56468-v • 09:37 min • March 15th, 2018
March 15th, 2018 •
*1, *2, *1, 3, 2, 1, 2, 1
1Department of Pediatric Dentistry, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Center of Stomatology, 2State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, 3Department of Oral and Maxillofacial-Head and Neck Oncology, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine
* These authors contributed equally

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本稿では、マウスの骨髄から破骨細胞形成の複雑な 1 ラパマイシンの哺乳類/機構のターゲットの役割を研究する分離と培養破骨細胞の in vitroにプロトコルについて説明します。

Tags

発生生物学、問題 133、破骨細胞、mTORC1、猛禽類、骨・細胞生物学、RANKL、M-CSF 修繕
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