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Sporing Drosophila larver atferd som svar til Optogenetic stimulering av Olfactory nerveceller

Article DOI: 10.3791/57353-v • 06:49 min • March 21st, 2018
March 21st, 2018 •
1,2, 1, 1, 1, 1, 1,2, 1,2
1Department of Biology, MS-0314, University of Nevada, 2Integrated Neuroscience Graduate Program, University of Nevada

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Denne protokollen analyserer navigasjons virkemåten til Drosophila Larven svar på samtidige optogenetic stimulering av dets olfactory neurons. Lys 630 nm wavelength brukes til å aktivere individuelle olfactory neurons uttrykke en rød-skiftet kanal rhodopsin. Larver bevegelse registreres samtidig, registrert digitalt og analysert ved hjelp av tilpasset skrevet programvare.

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Nevrovitenskap problemet 133 Optogenetics atferd Drosophila luktesans Olfactory reseptor Nevron lukt reseptor
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