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Ex Vivo Imagem de cálcio para visualizar as respostas do cérebro a sinalização endócrina em Drosophila

Article DOI: 10.3791/57701-v • 06:49 min • June 2nd, 2018
June 2nd, 2018 •
1, 2
1Division of Biological Science, Graduate School of Science, Nagoya University, 2Department of Molecular Genetics, Institute of Life Science, Kurume University

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Este artigo descreve um protocolo para a ex vivo cálcio imagem latente do cérebro drosófila . Neste método, compostos naturais ou sintéticos podem ser aplicados para o buffer para testar sua capacidade de ativar neurônios particulares do cérebro.

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Neurociência edição 136 cálcio imaging GCaMP explante de cérebro hormônio peptídeo receptor G-proteína-acoplado Drosophila
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