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Journal / Bioengineering / Uso di High-Throughput Microbioreactor sistema…
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Bioengineering

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JoVE Journal Bioengineering
Uso di High-Throughput Microbioreactor sistema automatizzato per la produzione del modello IgG1 in cellule CHO
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Uso di High-Throughput Microbioreactor sistema automatizzato per la produzione del modello IgG1 in cellule CHO

Article DOI: 10.3791/58231-v • 08:15 min • September 28th, 2018
September 28th, 2018 •
1, 1, 1, 1, 1, 1, 1, 1, 1
1Center for Drug Evaluation and Research, Office of Product Quality, Office of Biotechnology Products, Division of Biotechnology Review and Research II, U.S. Food and Drug Administration

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Un protocollo dettagliato per il funzionamento simultaneo di 48 colture parallele sotto varie condizioni in un sistema di microbioreactor è presentato. Processo di coltura cellulare, raccolta e analisi di titolo dell'anticorpo successivi sono descritti.

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Bioingegneria problema 139 cellule di ovaio di criceto cinese di Micro-bioreattore delle cellule di cultura di Screening glicani anticorpo monoclonale cromatografia di esclusione di formato Multi-Angle Light Scattering.
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