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Journal / Medicine / 平滑肌细胞近系追踪小鼠晚期动脉粥样硬化病变细胞成分的定量分析…
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平滑肌细胞近系追踪小鼠晚期动脉粥样硬化病变细胞成分的定量分析
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平滑肌细胞近系追踪小鼠晚期动脉粥样硬化病变细胞成分的定量分析

Article DOI: 10.3791/59139-v • 09:06 min • February 20th, 2019
February 20th, 2019 •
1, 1, 2,3, 1,4
1Heart, Lung, Blood and Vascular Medicine Institute, University of Pittsburgh, 2Robert M. Berne Cardiovascular Research Center, University of Virginia, 3Department of Biochemistry and Molecular Genetics, University of Virginia, 4Division of Cardiology, University of Pittsburgh School of Medicine

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我们提出了一个标准化的方案来表征晚期小鼠动脉粥样硬化病变的细胞组成, 包括动物解剖、组织包埋、切片、染色和腕头动脉分析的系统方法从动脉粥样硬化平滑肌细胞谱系追踪小鼠。

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医学 第144期 动脉粥样硬化 动脉粥样硬化病变 臂头动脉 组织准备和切片 免疫组织化学 免疫荧光 共聚焦显微镜
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