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Immunology and Infection

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In Vitro ELISA Test for å evaluere Rabies vaksine styrke

Article DOI: 10.3791/59641-v • 09:04 min • May 11th, 2020
May 11th, 2020 •
1, 1,2
1Unit Antiviral Strategies, OIE Reference Laboratories for Rift Valley Fever Virus & Crimean Congo Hemorrhagic Fever Virus, Institut Pasteur, 2Institut Pasteur de Guinée, Conakry

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Her beskriver vi en indirekte ELISA sandwich immunocapture for å bestemme immungen glykoprotein innholdet i rabies vaksiner. Denne testen bruker et nøytraliserende monoklonalt antistoff (mAb-D1) som gjenkjenner glykoproteintrimmere. Det er et alternativ til in vivo NIH-testen for å følge konsistensen av vaksinepotens under produksjonen.

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Immunologi og infeksjon Utgave 159 Rabies vaksinestyrke NIH-test ELISA-metode mAb-D1 monoklonalt antistoff Trimers av glykoprotein Glykoproteininnhold
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