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Journal / Developmental Biology / Ein verbesserter grüner…
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Developmental Biology

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JoVE Journal Developmental Biology
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Ein verbesserter grüner Fluoreszenzprotein-basierter Assay zur Untersuchung des Neuriten-Auswuchses in primärneuronen

Article DOI: 10.3791/60031-v • 08:02 min • October 19th, 2019
October 19th, 2019 •
*1, *1,2, *1, 1
1School of Life Sciences, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, 2Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen
* These authors contributed equally

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In diesem Bericht beschreiben wir ein einfaches Protokoll zur Untersuchung des Neuritenauswüchss in embryonalen kortikalen Neuronen von Ratten durch Co-Transfecting mit EGFP und dem Protein von Interesse.

Tags

Entwicklungsbiologie Ausgabe 152 verbessertes grünes fluoreszierendes Protein ImageJ Mikroskopie Neuritenwachstum primäre Neuronen Transfektion
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