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Primäre Kultur der Neuronen isoliert von Embryonal Mouse Cerebellum

Article DOI: 10.3791/60168-v • 08:09 min • October 26th, 2019
October 26th, 2019 •
1,2, 1,2, 1,2, 1,2, 3, 1, 2,4, 1,2
1Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, 2The Children's Hospital Research Institute of Manitoba (CHRIM), Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, 3Department of Oral Biology, University of Illinois at Chicago, 4Department of Pathology, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba

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Die Durchführung von In-vitro-Experimenten, um in vivo-Bedingungen so angemessen wie möglich zu reflektieren, ist keine leichte Aufgabe. Die Verwendung von primären Zellkulturen ist ein wichtiger Schritt zum Verständnis der Zellbiologie in einem ganzen Organismus. Das bereitgestellte Protokoll beschreibt, wie man erfolgreich wachsen und Kultur embryonale Maus Kleinhirn Neuronen.

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Neurowissenschaften Ausgabe 152 Embryo Kleinhirn Neuron Purkinje-Zelle Primärkultur Maus
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