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JoVE Journal Developmental Biology
Analyse af hæmatopoietisk Stam Progenitorcellernes metabolisme
Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos
Journal (Developmental Biology)
Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos
Spatiotemporal Subcellular Manipulation of the Microtubule Cytoskeleton in the Living Preimplantation Mouse Embryo using Photostatins
Journal (Developmental Biology)
Spatiotemporal Subcellular Manipulation of the Microtubule Cytoskeleton in the Living Preimplantation Mouse Embryo using Photostatins
 
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Analyse af hæmatopoietisk Stam Progenitorcellernes metabolisme

Article DOI: 10.3791/60234-v • 12:20 min • November 9th, 2019
November 9th, 2019 •
1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3
1Nationwide Children's Hospital, 2The Ohio State University College of Medicine, 3The Ohio State University Comprehensive Cancer Center

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Hæmatopoietiske Stam progenitorceller (Hspc'er) overgang fra en lysende tilstand til en differentierings tilstand på grund af deres metaboliske plasticitet under dannelse af blod. Her præsenterer vi en optimeret metode til måling af mitokondrie respiration og glycolyse af Hspc'er.

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Udviklingsmæssige biologi hæmatopoietiske stamceller metabolisme mitokondriel respiration glykolyse ekstracellulær flux analyse ikke-vedlige celler
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