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JoVE Journal Developmental Biology
En lagdelt montering metode for utvidet tidsforløp Konfokalmikroskopi mikroskopi av hele sebrafisk embryo
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En lagdelt montering metode for utvidet tidsforløp Konfokalmikroskopi mikroskopi av hele sebrafisk embryo

Article DOI: 10.3791/60321-v • 08:55 min • January 14th, 2020
January 14th, 2020 •
1, 2, 2, 3,4, 1,4, 5
1Computational Biomedicine Lab, Texas Institute of Measurement Evaluation and Statistics, University of Houston, 2Institute of Biosciences and Technology, Texas A&M Health Science Center, 3Department of Biology and Biochemistry, Center for Nuclear Receptors and Cell Signaling, University of Houston, 4Department of Biosciences and Nutrition, Novum, Karolinska Institutet, 5Department of Intelligent Systems Engineering, Indiana University

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Denne artikkelen beskriver en metode for å montere skjøre sebrafisk embryo for utvidet tidsforløp konfokalmikroskopi mikroskopi. Denne rimelige metoden er enkel å utføre ved hjelp av vanlige glass-bunn mikroskopi retter for bildebehandling på noen invertert mikroskop. Monteringen utføres i lag av agarose i ulike konsentrasjoner.

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Utviklingsbiologi sebrafisk embryo konfokalmikroskopi mikroskopi tidsforløp montering transgene
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