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Investigando a quebra da barreira intestinal em organóides vivos
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Investigando a quebra da barreira intestinal em organóides vivos

Article DOI: 10.3791/60546-v • 07:18 min • March 26th, 2020
March 26th, 2020 •
1, 2, 1, 1, 2, 3, *1, *1,3
1Division of Molecular and Experimental Surgery, Department of Surgery, Translational Research Center, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Universitätsklinikum Erlangen, 2Department of Medicine 1, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Universitätsklinikum Erlangen, 3Optical Imaging Centre Erlangen (OICE), Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg
* These authors contributed equally

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Aqui descrevemos uma técnica para quantificar a integridade da barreira de pequenos organóides intestinais. O fato de o método ser baseado em organóides vivos permite a investigação sequencial de diferentes substâncias moduladoras de integridade de barreira ou combinações dos mesmos de forma resolvida pelo tempo.

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Medicina Edição 157 doença inflamatória intestinal gama de interferon organóides intestinais permeabilidade de barreira análise funcional microscopia organóide viva
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