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JoVE Journal Bioengineering
Isolering af voksne humane Dermal fibroblaster fra abdominal hud og generering af inducerede pluripotente stamceller ved hjælp af en ikke-integrerings metode
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Isolering af voksne humane Dermal fibroblaster fra abdominal hud og generering af inducerede pluripotente stamceller ved hjælp af en ikke-integrerings metode

Article DOI: 10.3791/60629-v • 10:52 min • January 19th, 2020
January 19th, 2020 •
*1, *1, 1, 1, 1, 1, 1, 1
1Department of Public Health, University of Naples Federico II
* These authors contributed equally

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Celle omprogrammering kræver indførelse af vigtige gener, som regulerer og vedligeholder pluripotente celle tilstand. Den beskrevne protokol muliggør dannelse af inducerede pluripotente stamceller (iPSCs) kolonier fra humane Dermal fibroblaster uden virale/integreretsmetoder, men ved hjælp af ikke-modificerede RNAs (NM-RNAs) kombineret med immun unddragelse faktorer, der reducerer cellulære forsvarsmekanismer.

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Bioteknik inducerede pluripotente stamceller (iPSCs) celle omprogrammering Dermal fibroblaster ikke-modificerede RNAs NM-RNAs integrationsfri metode cellekultur human regenerativ medicin
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