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JoVE Journal Chemistry
Kvantifisering av metall utvasking i immobilisert metal affinitet kromatografi
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Kvantifisering av metall utvasking i immobilisert metal affinitet kromatografi

Article DOI: 10.3791/60690-v • 05:35 min • January 17th, 2020
January 17th, 2020 •
1, 1, 2, 1
1Department of Chemistry and Biochemistry, James Madison University, 2Biophysics Graduate Program, Ohio State University

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Vi presenterer en analyse for enkel kvantifisering av metaller introdusert til prøver utarbeidet ved hjelp av immobilisert metall affinitet kromatografi. Metoden bruker hydroxynaphthol blå som fargemetrisk metall indikator og en UV-Vis spektrofotometer som detektoren.

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Kjemi immobilisert metall affinitet kromatografi hydroxynaphthol blå HNB ni-NTA metall forurensning hans-tag
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