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Transcriptome-Wide Profiling of Protein-RNA Interactions by Cross-Linking and Immunoprecipitation Mediated by FLAG-Biotin Tandem Purification

Article DOI: 10.3791/60730-v • 08:46 min • May 18th, 2020
May 18th, 2020 •
1,2, 1, 1
1Tsinghua-Peking Center for Life Sciences, School of Medicine and School of Life Sciences, Tsinghua University, 2Department of Molecular Biology, University of Texas Southwestern Medical Center

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Ici, nous présentons un protocole CLIP-seq modifié appelé FBIoCLIP-seq avec purification tandem FLAG-biotine pour déterminer les cibles arn des protéines liant l’ARN (RBPs) dans les cellules mammifères.

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Génétique Numéro 159 FBIoCLIP-seq biologie de l’ARN protéine liant l’ARN interaction protéine-ARN WDR43 LIN28
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