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Journal / Immunology and Infection / Produksjon og karakterisering av humane…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Produksjon og karakterisering av humane makrofager fra pluripotente stamceller
Promoting 3-D Aggregation of FACS Purified Thymic Epithelial Cells with EAK 16-II/EAKIIH6 Self-assembling Hydrogel
Journal (Immunology and Infection)
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Bioenergetics and the Oxidative Burst: Protocols for the Isolation and Evaluation of Human Leukocytes and Platelets
Journal (Immunology and Infection)
Bioenergetics and the Oxidative Burst: Protocols for the Isolation and Evaluation of Human Leukocytes and Platelets
 
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Produksjon og karakterisering av humane makrofager fra pluripotente stamceller

Article DOI: 10.3791/61038-v • 08:05 min • April 16th, 2020
April 16th, 2020 •
1,2, 1, 1, 1, 1, 2, 2, 1
1Centre for Regenerative Medicine, Scottish Centre for Regenerative Medicine, University of Edinburgh, 2Centre for Reproductive Health, The Queen's Medical Research Institute, University of Edinburgh

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Denne protokollen beskriver den robuste generasjonen makrofager fra humane induserte pluripotente stamceller, og metoder for deres påfølgende karakterisering. Celleoverflatemarkøruttrykk, genuttrykk og funksjonelle analyser brukes til å vurdere fenotypen og funksjonen til disse iPSC-avledede makrofagene.

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Immunologi og infeksjon Utgave 158 pluripotente stamceller differensiering myeloide celler makrofager fagocytose polarisering
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