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In Vitro evaluering af onkogene transformation i humane mammary epitelceller

Article DOI: 10.3791/61716-v • 09:44 min • September 24th, 2020
September 24th, 2020 •
1,2, 1,3,4, 5,6,7, 1, 1
1Department of Cell Biology, Physiology and Immunology, Universitat Autònoma de Barcelona, 2Optical Microscopy Core Facilities, IDIBELL, 3Hereditary Cancer Program, Catalan Institute of Oncology, IDIBELL, 4Program in Molecular Mechanisms and Experimental Therapy in Oncology (Oncobell), IDIBELL, 5New Therapeutic Strategies in Cancer Group. Department of Biochemistry and Molecular Biomedicine, School of Biology, Institute of Biomedicine, University of Barcelona (IBUB), 6Department of Biochemistry & Physiology, School of Pharmacy and Food Sciences, University of Barcelona, 7Department of Biosciences, Faculty of Sciences and Technology, University of Vic

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Denne protokol giver eksperimentelle in vitro værktøjer til at evaluere omdannelsen af menneskelige mælkeceller. Detaljerede trin til opfølgende celleproliferedningshastighed, forankrings-uafhængig vækstkapacitet og fordeling af cellestælg i 3D-kulturer med kældermembranmatrix er beskrevet.

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KræftForskning cellulære transformation vækstkurve forankring assay 3D celle kultur menneskelige bryst epitelceller tumorigenesis
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