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Cancer Research

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JoVE Journal Cancer Research
Fluorescentiemicroscopie voor ATP-internalisatie gemedieerd door macropinocytose in menselijke tumorcellen en tumor-xenogetransplanteerde muizen
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Fluorescentiemicroscopie voor ATP-internalisatie gemedieerd door macropinocytose in menselijke tumorcellen en tumor-xenogetransplanteerde muizen

Article DOI: 10.3791/62768-v • 08:31 min • June 30th, 2021
June 30th, 2021 •
*1,2,3, *4, 1,5, 4, 1,2,4, 1,2,4, 6, 1,2,4,7
1Department of Biological Sciences, Ohio University, 2Molecular and Cellular Biology Program, Ohio University, 3Neuroscience Program, Ohio University, 4Edison Biotechnology Institute, Ohio University, 5Translational Biomedical Sciences Program, Ohio University, 6Honors Tutorial College, Ohio University, 7Department of Biomedical Sciences, Heritage College of Osteopathic Medicine, Ohio University
* These authors contributed equally

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We ontwikkelden een reproduceerbare methode om de internalisatie van niet-hydrolyseerbaar fluorescerend adenosinetrifosfaat (ATP), een ATP-surrogaat, met hoge cellulaire resolutie te visualiseren. We hebben onze methode gevalideerd met behulp van onafhankelijke in vitro en in vivo assays - menselijke tumorcellijnen en immunodeficiënte muizen xenografeerd met menselijk tumorweefsel.

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Kankeronderzoek Nummer 172
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