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Kärnisolering från vuxen musnjure för RNA-sekvensering med en kärna
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Kärnisolering från vuxen musnjure för RNA-sekvensering med en kärna

Article DOI: 10.3791/62901-v • 06:00 min • September 20th, 2021
September 20th, 2021 •
*1,2, *1,2, 3, 4, 3, 3, 1,2
1Department of Nephrology and Medical Intensive Care Medicine, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 2Molecular and Translational Kidney Research, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 3Berlin Institute of Medical Systems Biology (BIMSB), Systems Biology of Gene Regulatory Elements, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 4Berlin Institute of Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC)
* These authors contributed equally

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Här presenterar vi ett protokoll för att isolera högkvalitativa kärnor från frysta musnjurar som förbättrar representationen av medullära njurcelltyper och undviker genuttrycksartefakter från enzymatisk vävnadsdissociation.

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Biologi utgåva 175 njure kärnisolering transkriptomik transkriptomsekvensering RNA-sekvensering med en kärna snRNA-seq
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