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Microtubule Plus-End Dynamics Visualisering i Huntingtons sjukdom modell baserat på mänskliga primära hud fibroblaster
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Microtubule Plus-End Dynamics Visualisering i Huntingtons sjukdom modell baserat på mänskliga primära hud fibroblaster

Article DOI: 10.3791/62963-v • 10:38 min • January 8th, 2022
January 8th, 2022 •
*1,2, *2,3, 1,2, 3,4, 3,4, 1,3
1A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 2Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 3Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 4Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
* These authors contributed equally

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Detta protokoll är dedikerat till microtubule plus-end visualisering av EB3 proteintransfektion för att studera deras dynamiska egenskaper i primärcellskultur. Protokollet genomfördes på mänskliga primära huden fibroblaster som erhållits från Huntingtons sjukdom patienter.

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Bioengineering nummer 179 mikrotubuli transfektion EB3 primärkultur Huntingtons sjukdom livstidsmikroskopi
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