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Neuroscience

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JoVE Journal Neuroscience
Live Imaging af Mitokondrie Glutathione Redox tilstand i primære neuroner ved hjælp af en ratiometrisk indikator
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Live Imaging af Mitokondrie Glutathione Redox tilstand i primære neuroner ved hjælp af en ratiometrisk indikator

Article DOI: 10.3791/63073-v • 07:47 min • October 20th, 2021
October 20th, 2021 •
1, 1, 2, 1
1Department of Medical Cell Biology, Institute for Anatomy and Cell Biology, Heidelberg University, 2Department of Neurogenetics, Max-Planck-Institute for Experimental Medicine

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Denne artikel beskriver en protokol til at bestemme forskelle i basal redox tilstand og redox svar på akutte forstyrrelser i primære hippocampal og kortikale neuroner ved hjælp af konfokal levende mikroskopi. Protokollen kan anvendes på andre celletyper og mikroskoper med minimale ændringer.

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Neurovidenskab Grx1-roGFP2 konfokal mikroskopi oxidativ stress hippocampal neuroner excitotoxicity mitokondriemembran potentiale
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