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生物化学
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Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

DOI:
10.3791/64200-v

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09:49 min

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November 18, 2022

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, , , ,
1Department of Chemistry,University of Florida, 2Division of Protective Immunity,Children’s Hospital of Philadelphia, 3Department of Pathology and Laboratory Medicine, Perelman School of Medicine,University of Pennsylvania

Chapters

  • 00:05Introduction
  • 00:58Ambient, Vacuum, and Heat-Mediated Drying of Clostridioides difficile and Enterococcus faecalis Bacterial Macrocolonies
  • 02:33MALDI Matrix Application via Robotic Spraying
  • 03:12Preparing Bacterial Macrocolonies for MALDI Imaging Mass Spectrometry Data Acquisition
  • 04:37Imaging Mass Spectrometry Data Analysis and Compound Identification
  • 05:57Cryosectioning of Noninfected Control Versus Clostridioides difficile-Infected Mouse Cecal Tissues
  • 07:45Preparation of Noninfected Control Versus Clostridioides difficile-Infected Mouse Cecal Tissues for Matrix Application and MALDI Imaging Mass Spectrometry
  • 08:08Results: Imaging Mass Spectrometry Analysis of Bacterial Macrocolonies and Infected Murine Cecal Tissues
  • 09:09Conclusion

Summary

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A novel sample preparation method is demonstrated for the analysis of agar-based, bacterial macrocolonies via matrix-assisted laser desorption/ionization imaging mass spectrometry.

Tags

Microbial CooperationImaging Mass SpectrometryBacterial ColoniesAgarMALDI MatrixSample PreparationVacuum DryingSpatial MetabolismMetabolitesMicrobial Pathogens

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