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Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection
DOI:

09:49 min

November 18, 2022

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Chapters

  • 00:05Introduction
  • 00:58Ambient, Vacuum, and Heat-Mediated Drying of Clostridioides difficile and Enterococcus faecalis Bacterial Macrocolonies
  • 02:33MALDI Matrix Application via Robotic Spraying
  • 03:12Preparing Bacterial Macrocolonies for MALDI Imaging Mass Spectrometry Data Acquisition
  • 04:37Imaging Mass Spectrometry Data Analysis and Compound Identification
  • 05:57Cryosectioning of Noninfected Control Versus Clostridioides difficile-Infected Mouse Cecal Tissues
  • 07:45Preparation of Noninfected Control Versus Clostridioides difficile-Infected Mouse Cecal Tissues for Matrix Application and MALDI Imaging Mass Spectrometry
  • 08:08Results: Imaging Mass Spectrometry Analysis of Bacterial Macrocolonies and Infected Murine Cecal Tissues
  • 09:09Conclusion

Summary

自动翻译

A novel sample preparation method is demonstrated for the analysis of agar-based, bacterial macrocolonies via matrix-assisted laser desorption/ionization imaging mass spectrometry.

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