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JoVE Journal Cancer Research
Co-kultur og transduktion af murine thymocytter på delta-lignende 4-ekspression stromale celler til undersøgelse af onkogener i T-celle leukæmi
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Co-kultur og transduktion af murine thymocytter på delta-lignende 4-ekspression stromale celler til undersøgelse af onkogener i T-celle leukæmi

Article DOI: 10.3791/64271-v • 09:43 min • June 9th, 2023
June 9th, 2023 •
1, 1, 1,2,3, 1, 1,2,3, 4, 1, 1
1Cytokines and Immunity Section, Cancer Innovation Laboratory, National Cancer Institute, National Institutes of Health, 2Comparative Biomedical Scientist Training Program, NIH, 3Department of Pathobiology and Diagnostic Investigation, Veterinary Diagnostic Laboratory, Michigan State University, 4NCI-Frederick Laboratory Animal Sciences Program

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Denne protokol beskriver isoleringen af dobbelt-negative thymocytter fra musethymus efterfulgt af retroviral transduktion og co-kultur på det deltalignende 4-ekspressive knoglemarvstromale cellelinje-cokultursystem (OP9-DL4) til yderligere funktionel analyse.

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Kræftforskning udgave 196 thymocyt OP9 lymfopoieser transduktion co-kultur
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