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JoVE Journal Cancer Research
Co-coltura e trasduzione di timociti murini su cellule stromali esprimenti delta-like 4 per studiare oncogeni nella leucemia a cellule T
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Co-coltura e trasduzione di timociti murini su cellule stromali esprimenti delta-like 4 per studiare oncogeni nella leucemia a cellule T

Article DOI: 10.3791/64271-v • 09:43 min • June 9th, 2023
June 9th, 2023 •
1, 1, 1,2,3, 1, 1,2,3, 4, 1, 1
1Cytokines and Immunity Section, Cancer Innovation Laboratory, National Cancer Institute, National Institutes of Health, 2Comparative Biomedical Scientist Training Program, NIH, 3Department of Pathobiology and Diagnostic Investigation, Veterinary Diagnostic Laboratory, Michigan State University, 4NCI-Frederick Laboratory Animal Sciences Program

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Questo protocollo descrive l'isolamento dei timociti doppi negativi dal timo di topo seguito da trasduzione retrovirale e co-coltura sul sistema di co-coltura della linea cellulare stromale del midollo osseo a 4 esprimenti delta (OP9-DL4) per ulteriori analisi funzionali.

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Ricerca sul cancro numero 196 timocita OP9 linfopoiesi trasduzione co-coltura
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