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JoVE Journal Biology
Isolering af hvilende stamcellepopulationer fra individuelle skeletmuskler
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Isolering af hvilende stamcellepopulationer fra individuelle skeletmuskler

Article DOI: 10.3791/64557-v • 11:35 min • December 9th, 2022
December 9th, 2022 •
*1, *1, 1, 1, 1
1Department of Biomedicine, Aarhus University
* These authors contributed equally

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Denne protokol beskriver isoleringen af muskelstamceller og fibro-adipogene forfædre fra individuelle skeletmuskler hos mus. Protokollen involverer enkelt muskeldissektion, stamcelleisolering ved fluorescensaktiveret cellesortering, renhedsvurdering ved immunofluorescensfarvning og kvantitativ måling af S-faseindgang ved 5-ethynyl-2'-deoxyuridininkorporeringsassay.

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Tilbagetrækning nummer 190
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