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Effektiv PAM-mindre baseredigering for sebrafiskmodellering av menneskelig genetisk sykdom med zSpRY-ABE8e

Article DOI: 10.3791/64977-v • 07:31 min • February 17th, 2023
February 17th, 2023 •
1,2, 3, 1,2, 4, 1,2, 1,2
1Key Laboratory of Brain, Cognition and Education Science, Ministry of Education, 2Institute for Brain Research and Rehabilitation, Guangdong Key Laboratory of Mental Health and Cognitive Science, South China Normal University, 3Institute of Modern Aquaculture Science and Engineering, Guangdong Provincial Engineering Technology Research Center for Environmentally Friendly Aquaculture, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Sciences, South China Normal University, 4Department of Pathology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences

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Denne protokollen beskriver hvordan man utfører effektiv adeninbaseredigering uten PAM-begrensning for å konstruere en presis sebrafisksykdomsmodell ved bruk av zSpRY-ABE8e.

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