Expression and Purification of Human Solute Carrier Transporters Using Codon-Optimized Genes (Video) | JoVE

Tags

High-Throughput Expression Purification Human Solute Carriers Structural Studies Biochemical Studies Membrane Transporters Endogenous Substrates Exogenous Substrates Therapeutic Targets Markers Of Disease Pharmaceuticals Drug Discovery Projects Limited Structural Knowledge Limited Functional Knowledge Limited Physiological Knowledge Expression And Purification Difficulties Milligram Quantities Codon-optimized Gene Sequences Construct Design High-throughput Expression Structural Integrity Preservation Biochemical Activity Preservation Eukaryotic Cell Expression Affinity Purification Size-exclusion Chromatography High-resolution Structure Determination Transport Studies, Small-molecule Engagement Assays High-throughput In Vitro Screening