Anesthetized Mouse Model
Anesthetized Mouse Model - Related Videos
04:17
Electrophoretic Delivery of GABA into the Epileptic Focus of an Anesthetized Mouse
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2025
Source: Slezia, A. et al. Electrophoretic Delivery of γ-aminobutyric Acid (GABA) into Epileptic Focus Prevents Seizures in Mice. J. Vis. Exp. (2019)This video demonstrates the electrophoretic delivery of gamma-aminobutyric acid (GABA) to inhibit seizure activity in an anesthetized mouse. It outlines the steps involved in probe implantation, neural activity recording, seizure induction, and controlled GABA delivery via a microfluidic ion pump to assess its therapeutic efficacy.
06:38
Use of a Piglet Model for the Study of Anesthetic-induced Developmental Neurotoxicity (AIDN): A Translational Neuroscience Approach
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Cited by 11 •
2017
Anesthesia-induced developmental neurotoxicity (AIDN) research has focused on rodents, which are not broadly applicable to humans. Non-human primate models are more relevant, but are cost-prohibitive and difficult to use for experimentation. The piglet, in contrast, is a clinically relevant, practical animal model ideal for the study of anesthetic neurotoxicity.
06:24
Mouse Models of Periventricular Leukomalacia
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Cited by 28 •
2010
We established mouse models of periventricular leukomalacia (PVL), the predominant brain injury in premature infants characterized by periventricular white matter lesions. Hypoxia/ischemia with/without systemic infection are the primary causes of PVL. Unilateral carotid ligation and hypoxia exposure with/without lipopolysaccharide injection creates PVL-like lesions in P6 mice.
08:43
Orthotopic Mouse Model of Colorectal Cancer
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Cited by 109 •
2007
Two techniques can be used to establish this model: injection of a cancer cell suspension into the cecal wall or transplantation of a piece of subcutaneous tumor onto the cecum. This model is useful for studying the natural progression of colorectal cancer and testing new therapeutic agents against colorectal cancer.
01:20
Inhalational Anesthetics: Overview
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2024
Inhalation anesthetics are drugs that induce general anesthesia upon inhalation. They work by increasing the sensitivity of GABAA receptors or inhibiting NMDA receptors, leading to a decrease in central nervous system activity. The depth of anesthesia can be rapidly adjusted by changing the concentration of the inhaled gas. Some common examples of inhalational anesthetics include volatile liquids like isoflurane, desflurane, sevoflurane and gases like xenon and nitrous oxide. Isoflurane, a...
FAQs
Anesthetic depth must be adjusted to the procedure and maintained consistently throughout the session. It determines whether the mouse remains sufficiently immobile and has reduced pain responses while researchers perform an intervention, yet the preparation must also remain physiologically stable. Recording and controlling depth helps separate treatment- or tumor-related findings from variability introduced by anesthesia.
Temperature and respiration are key physiological variables during an anesthetized mouse model experiment. Monitoring them helps researchers recognize changes that could disturb the stable state required for tumor procedures, imaging, sampling, or surgery. Recovery also matters: documenting the return from anesthesia supports animal welfare and helps researchers interpret observations made during and after the experimental intervention.
Reducing stress and discomfort improves the consistency of experimental conditions while supporting responsible animal research. In cancer studies, this matters because measurements such as tumor growth, treatment response, metastasis, and cancer-associated physiology are intended to reflect the disease or intervention under study. Controlled anesthesia helps prevent avoidable distress from becoming an uncontrolled source of experimental variation.
A typical workflow begins with administration of anesthetic agents under monitored conditions, followed by confirmation that the mouse is in an appropriate stable state for the planned procedure. Researchers then perform tumor implantation, imaging, tissue sampling, or surgery while controlling anesthetic depth, temperature, and respiration. Afterward, they monitor recovery rather than ending observation when the procedure is complete.
This model is useful whenever cancer experiments require reduced movement and pain responses during a controlled intervention. Examples include establishing tumors through implantation, acquiring tissue samples, conducting imaging, and performing surgery. The preparation can also support assessments of tumor growth, treatment response, metastasis, and cancer-associated physiology, depending on the measurements and procedures included in the study.
Researchers can evaluate tumor growth, responses to treatment, metastatic processes, and cancer-associated physiology using procedures conducted under controlled anesthesia. Imaging, tissue sampling, implantation, and surgery provide different ways to examine these outcomes. Reliable interpretation depends on maintaining anesthetic depth and physiological conditions consistently, because uncontrolled changes in temperature, respiration, or recovery can complicate experimental comparisons.